APPLICATION NOTE
Infant Nutritional Formula – NIST 1849b
SUMMARY
The application note summarizes the digestion of NIST 1849b, an infant nutritional formula standard reference material using
ColdBlock™ Digestion Pro Series Technology
| Instrument: | ColdBlock CBM (with quartz test tubes), chiller, ICP-MS |
| Published: | May 2024 |
| Digestion Time: | 20 Minutes |
| Acid Used: | HNO3 & H2O2 |
| Average ColdBlock Recovery vs. CRM: |
|
METHODOLOGY
- Chiller temperature was set to -5°C
- 0.5g of each sample was weighed and placed into a quartz ColdBlock™ Digestion vessel
- 10 mL of HNO3 was added and mixed with the sample
- Sample was digested at 65% power for 20 minutes
- 2mL of ≥ 30% H2O2 was added
- Samples were cooled and bulked to 40mL using 2% HNO3 v/v
DISCUSSION
- Samples were digested in triplicate
- Samples were filtered prior to analysis by ICP-MS
- H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%,
for trace analysis) - HNO3 (Analytichem 250-038-175, Nitric Acid, PP, 67-70%)
- NIST 1849b is a milk-based, hybrid infant/adult nutritional powder, prepared by a manufacturer of infant formula and adult nutritional products
NIST 1849b after bulk-up to 40mL
Results
|
NIST 1849b – Adult/Infant Nutritional Formula I |
||||||||||
|
Method: |
0.5g |
10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2, let cool and bulked to 40mL with 2% HNO3 v/v |
||||||||
|
Element |
NIST Certified Values (mg/kg) |
95% Confidence Limits |
Sample A |
Sample B |
Sample C |
Average (mg/kg) |
Stdev |
% RSD |
% Recovery |
|
|
Low |
High |
|||||||||
|
Cr |
1.033 |
1.015 |
1.051 |
0.913 |
1.005 |
0.926 |
0.948 |
0.041 |
4.3% |
92% |
|
Cu |
18.96 |
18.66 |
19.26 |
18.93 |
18.69 |
18.29 |
18.64 |
0.3 |
1.4% |
98% |
|
Fe |
168 |
160.6 |
175.4 |
174 |
174 |
161 |
170 |
6.4 |
3.8% |
101% |
|
Mg |
1570 |
1540 |
1600 |
1531 |
1599 |
1518 |
1549 |
36 |
2.3% |
99% |
|
Mo |
1.741 |
1.678 |
1.804 |
1.764 |
1.766 |
1.740 |
1.757 |
0.012 |
0.7% |
101% |
|
P |
3750 |
3620 |
3880 |
3802 |
3900 |
3952 |
3885 |
62 |
1.6% |
104% |
|
Se |
0.816 |
0.791 |
0.841 |
0.759 |
0.802 |
0.822 |
0.794 |
0.026 |
3.3% |
97% |
|
*Ca |
5050 |
4830 |
5270 |
5122 |
4975 |
4805 |
4968 |
129 |
2.6% |
98% |
|
*Mn |
46.4 |
44.3 |
48.5 |
44.1 |
42.9 |
43.2 |
43.4 |
0.5 |
1.2% |
94% |
|
*K |
9014 |
8924 |
9104 |
9035 |
8723 |
8964 |
8907 |
133 |
1.5% |
99% |
|
*Na |
4155 |
4055 |
4255 |
5127 |
4103 |
4277 |
4502 |
448 |
9.9% |
108% |
|
*Zn |
141.9 |
138 |
145.8 |
161.1 |
147.1 |
138.6 |
148.9 |
9.3 |
6.2% |
105% |
Click here to download the Application Note in PDF format.
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4551 Zimmerman Ave
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info@coldblock.ca.