Infant Nutritional Formula - NIST 1849b

APPLICATION NOTE

Infant Nutritional Formula – NIST 1849b

SUMMARY

The application note summarizes the digestion of NIST 1849b, an infant nutritional formula standard reference material using ColdBlock Digestion Pro Series Technology

Instrument:	ColdBlock CBM (with quartz test tubes), chiller, ICP-MS
Published:	May 2024
Digestion Time:	20 Minutes
Acid Used:	HNO3 & H2O2
Average ColdBlock Recovery vs. CRM:	101% Iron 101% Molybdenum 97% Selenium

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METHODOLOGY

Chiller temperature was set to -5°C
0.5g of each sample was weighed and placed into a quartz ColdBlock Digestion vessel
10 mL of HNO₃ was added and mixed with the sample
Sample was digested at 65% power for 20 minutes
2mL of ≥ 30% H₂O₂ was added
Samples were cooled and bulked to 40mL using 2% HNO_{3 v/v}

DISCUSSION

Samples were digested in triplicate
Samples were filtered prior to analysis by ICP-MS
H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%,
for trace analysis)
HNO3 (Analytichem 250-038-175, Nitric Acid, PP, 67-70%)
NIST 1849b is a milk-based, hybrid infant/adult nutritional powder, prepared by a manufacturer of infant formula and adult nutritional products

NIST 1849b after bulk-up to 40mL

Results

NIST 1849b – Adult/Infant Nutritional Formula I
Method:	0.5g	10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2, let cool and bulked to 40mL with 2% HNO3 v/v
Element	NIST Certified Values (mg/kg)	95% Confidence Limits		Sample A	Sample B	Sample C	Average (mg/kg)	Stdev	% RSD	% Recovery
Element	NIST Certified Values (mg/kg)	Low	High	Sample A	Sample B	Sample C	Average (mg/kg)	Stdev	% RSD	% Recovery
Cr	1.033	1.015	1.051	0.913	1.005	0.926	0.948	0.041	4.3%	92%
Cu	18.96	18.66	19.26	18.93	18.69	18.29	18.64	0.3	1.4%	98%
Fe	168	160.6	175.4	174	174	161	170	6.4	3.8%	101%
Mg	1570	1540	1600	1531	1599	1518	1549	36	2.3%	99%
Mo	1.741	1.678	1.804	1.764	1.766	1.740	1.757	0.012	0.7%	101%
P	3750	3620	3880	3802	3900	3952	3885	62	1.6%	104%
Se	0.816	0.791	0.841	0.759	0.802	0.822	0.794	0.026	3.3%	97%
*Ca	5050	4830	5270	5122	4975	4805	4968	129	2.6%	98%
*Mn	46.4	44.3	48.5	44.1	42.9	43.2	43.4	0.5	1.2%	94%
*K	9014	8924	9104	9035	8723	8964	8907	133	1.5%	99%
*Na	4155	4055	4255	5127	4103	4277	4502	448	9.9%	108%
*Zn	141.9	138	145.8	161.1	147.1	138.6	148.9	9.3	6.2%	105%

Click here to download the Application Note in PDF format.

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