APPLICATION NOTE
Meat Homogenate – NIST 1546a
SUMMARY
The application note summarizes the digestion of NIST 1546a, a meat homogenate standard reference material using ColdBlock™ Digestion Pro Series Technology
| Instrument: | ColdBlock CBM (with quartz test tubes), chiller, ICP-MS |
| Published: | May 2024 |
| Digestion Time: | 20 Minutes |
| Acid Used: | HNO3& H2O2 |
| Average ColdBlock Recovery vs. CRM: |
|
METHODOLOGY
- Chiller temperature was set to -5°C
- 0.5g of each sample was weighed and placed into a quartz
ColdBlock™ Digestion vessel - 10 mL of HNO3
was added and mixed with the sample - Sample was digested at 65% power for 20 minutes
- 2mL of ≥ 30% H2O2 was added
- Samples were cooled and bulked to 40mL using 2% HNO3 v/v
DISCUSSION
- Samples were digested in triplicate
- Samples were filtered prior to analysis by ICP-MS
- H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%, for trace analysis)
- HNO3 (Analytichem, 250-038-175, Nitric Acid, PP, 67-70%)
- NIST 1546a consists of a mixture of pork and chicken products
blended in a commercial process
NIST 1546a after bulk-up to 40mL
Results
|
NIST 1546a – Meat Homogenate |
||||||||||
|
Method: |
0.5g |
10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2, let cool and bulked to 40mL with 2% HNO3 v/v |
||||||||
|
Element |
NIST Certified Values (mg/kg) |
95% Confidence Limits |
Sample A |
Sample B |
Sample C |
Average (mg/kg) |
Stdev |
% RSD |
% Recovery |
|
|
Low |
High |
|||||||||
|
Cu |
0.605 |
0.554 |
0.656 |
0.671 |
0.532 |
0.656 |
0.620 |
0.062 |
10.1% |
102% |
|
Fe |
10.17 |
9.82 |
10.52 |
9.86 |
11.06 |
10.29 |
10.40 |
0.50 |
4.8% |
102% |
|
Mg |
178.1 |
173.3 |
182.9 |
186.6 |
160.8 |
169.3 |
172.2 |
10.8 |
6.2% |
97% |
|
Mn |
0.286 |
0.262 |
0.31 |
0.272 |
0.257 |
0.270 |
0.266 |
0.007 |
2.5% |
93% |
|
P |
1651 |
1619 |
1683 |
1583 |
1681 |
1806 |
1690 |
92 |
5.4% |
102% |
|
K |
2490 |
2280 |
2700 |
2409 |
2720 |
2536 |
2555 |
128 |
5.0% |
103% |
|
Se |
0.281 |
0.264 |
0.298 |
0.273 |
0.308 |
0.303 |
0.295 |
0.015 |
5.2% |
105% |
|
Na |
9600 |
8500 |
10700 |
9677 |
8931 |
9562 |
9390 |
328 |
3.5% |
98% |
|
Zn |
17.88 |
17.53 |
18.23 |
18.94 |
17.29 |
18.39 |
18.21 |
0.69 |
3.8% |
102% |
|
*Ba |
0.077 |
0.058 |
0.096 |
0.078 |
0.071 |
0.065 |
0.071 |
0.005 |
7.4% |
93% |
|
*B |
0.306 |
0.267 |
0.345 |
0.257 |
0.273 |
0.310 |
0.280 |
0.022 |
7.9% |
92% |
|
*Ca |
360 |
230 |
490 |
334 |
439 |
366 |
378 |
44 |
11.6% |
105% |
|
*Mo |
0.016 |
0.014 |
0.018 |
0.014 |
0.017 |
0.017 |
0.016 |
0.001 |
8.8% |
100% |
|
*Rb |
2.56 |
2.45 |
2.67 |
2.57 |
2.81 |
2.42 |
2.60 |
0.16 |
6.2% |
102% |
|
*Sr |
0.305 |
0.235 |
0.375 |
0.309 |
0.368 |
0.317 |
0.331 |
0.03 |
7.9% |
109% |
Click here to download the Application Note in PDF format.
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4551 Zimmerman Ave
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info@coldblock.ca.