Meat Homogenate - NIST 1546a - ColdBlock Technologies

APPLICATION NOTE

Meat Homogenate – NIST 1546a

SUMMARY

The application note summarizes the digestion of NIST 1546a, a meat homogenate standard reference material using ColdBlock Digestion Pro Series Technology

Instrument:	ColdBlock CBM (with quartz test tubes), chiller, ICP-MS
Published:	May 2024
Digestion Time:	20 Minutes
Acid Used:	HNO₃& H₂O₂
Average ColdBlock Recovery vs. CRM:	102% Copper 104% Iron 105% Selenium

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METHODOLOGY

Chiller temperature was set to -5°C
0.5g of each sample was weighed and placed into a quartz
ColdBlock Digestion vessel
10 mL of HNO3
was added and mixed with the sample
Sample was digested at 65% power for 20 minutes
2mL of ≥ 30% H2O2 was added
Samples were cooled and bulked to 40mL using 2% HNO3 v/v

DISCUSSION

Samples were digested in triplicate
Samples were filtered prior to analysis by ICP-MS
H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%, for trace analysis)
HNO3 (Analytichem, 250-038-175, Nitric Acid, PP, 67-70%)
NIST 1546a consists of a mixture of pork and chicken products
blended in a commercial process

NIST 1546a after bulk-up to 40mL

Results

NIST 1546a – Meat Homogenate
Method:	0.5g	10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2, let cool and bulked to 40mL with 2% HNO3 v/v
Element	NIST Certified Values (mg/kg)	95% Confidence Limits		Sample A	Sample B	Sample C	Average (mg/kg)	Stdev	% RSD	% Recovery
Element	NIST Certified Values (mg/kg)	Low	High	Sample A	Sample B	Sample C	Average (mg/kg)	Stdev	% RSD	% Recovery
Cu	0.605	0.554	0.656	0.671	0.532	0.656	0.620	0.062	10.1%	102%
Fe	10.17	9.82	10.52	9.86	11.06	10.29	10.40	0.50	4.8%	102%
Mg	178.1	173.3	182.9	186.6	160.8	169.3	172.2	10.8	6.2%	97%
Mn	0.286	0.262	0.31	0.272	0.257	0.270	0.266	0.007	2.5%	93%
P	1651	1619	1683	1583	1681	1806	1690	92	5.4%	102%
K	2490	2280	2700	2409	2720	2536	2555	128	5.0%	103%
Se	0.281	0.264	0.298	0.273	0.308	0.303	0.295	0.015	5.2%	105%
Na	9600	8500	10700	9677	8931	9562	9390	328	3.5%	98%
Zn	17.88	17.53	18.23	18.94	17.29	18.39	18.21	0.69	3.8%	102%
*Ba	0.077	0.058	0.096	0.078	0.071	0.065	0.071	0.005	7.4%	93%
*B	0.306	0.267	0.345	0.257	0.273	0.310	0.280	0.022	7.9%	92%
*Ca	360	230	490	334	439	366	378	44	11.6%	105%
*Mo	0.016	0.014	0.018	0.014	0.017	0.017	0.016	0.001	8.8%	100%
*Rb	2.56	2.45	2.67	2.57	2.81	2.42	2.60	0.16	6.2%	102%
*Sr	0.305	0.235	0.375	0.309	0.368	0.317	0.331	0.03	7.9%	109%

Click here to download the Application Note in PDF format.

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