Corn Bran - NRC BRAN-1 - ColdBlock Technologies

APPLICATION NOTE

Corn Bran – NRC BRAN-1

SUMMARY

The application note summarizes the digestion of BRAN-1, a Corn Bran Reference Material using ColdBlock Digestion Pro Series Technology.

Instrument:	ColdBlock CBM sample digester, chiller & ICP-MS
Published:	March 2023
Digestion Time:	10 Minutes
Acid Used:	HNO₃ & H₂O₂
Average ColdBlock Recovery vs. CRM:	94% Cadmium 114% Lead 99% Selenium

METHODOLOGY

Chiller temperature was set to -5°C
0.5g of each sample was weighed and placed into a ColdBlock Digestion vessel
15 mL of HNO₃ + 2mL H₂O₂ was added
Sample was digested at 70% power for 10 minutes
Samples were cooled and bulked to 40mL using 2% HNO₃ + 0.5% HCl_v/v

DISCUSSION

A minor of amount of material settled on the bottom of the tube after bulking
Samples were filtered prior to analysis

BRAN-1 is a Reference Material prepared from refined corn bran obtained from A.E Staley Manufacturing Co., Decatur, IL USA. BRAN-1, formerly known as RM 8433 was prepared by the Centre for Land and Biological resources Research, Agriculture Canada. BRAN-1; Corn Bran; Reference Material; National Research Council Canada; Ottawa, Ontario Canada (September 2015)

Results

NRC BRAN-1 (Corn Bran)
Method:	0.5g	15mL HNO3 + 2mL H2O2 Digest at 60% power for 10 minutes.
Element	Reference Value (mg/kg)	95% Confidence Limits		Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
Element	Reference Value (mg/kg)	Low	High	Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
Ca	420	382	458	326	486	461	424	70.349	16.6%	101%
Cd	0.012	0.007	0.017	0.011	0.011	0.012	0.011	0.0005	4.2%	94%
Cu	2.47	2.07	2.87	2.56	2.69	2.68	2.65	0.058	2.2%	107%
Fe	14.8	13	16.6	15.3	16.5	15.9	15.9	0.469	3.0%	107%
K	566	491	641	600	606	600	602	2.676	0.4%	106%
Mg	818	759	877	802	781	786	789	9.010	1.1%	97%
Mn	2.55	2.26	2.84	2.52	2.59	2.55	2.55	0.031	1.2%	100%
Na	430	399	461	432	422	433	429	4.955	1.2%	100%
Pb	0.14	0.106	0.174	0.147	0.17	0.163	0.16	0.010	6.0%	114%
Rb	0.5	0.2	0.8	0.5	0.5	0.5	0.5	0.030	6.3%	97%
Se	0.045	0.037	0.053	0.056	0.036	0.042	0.045	0.008	18.8%	99%
Sr	4.6	N/A	N/A	5.164	5.24	5.075	5.2	0.067	1.3%	112%
Zn	18.6	16.4	20.8	19.609	19.178	20.976	19.9	0.766	3.8%	107%

Click here to download the Application Note in PDF format.

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