APPLICATION NOTE
Corn Bran – NRC BRAN-1
SUMMARY
The application note summarizes the digestion of BRAN-1, a Corn Bran Reference Material using ColdBlock™ Digestion Pro Series Technology.
| Instrument: | ColdBlock CBM sample digester, chiller & ICP-MS |
| Published: | March 2023 |
| Digestion Time: | 10 Minutes |
| Acid Used: | HNO3 & H2O2 |
| Average ColdBlock Recovery vs. CRM: |
|
METHODOLOGY
- Chiller temperature was set to -5°C
- 0.5g of each sample was weighed and placed into a ColdBlock™ Digestion vessel
- 15 mL of HNO3 + 2mL H2O2 was added
- Sample was digested at 70% power for 10 minutes
- Samples were cooled and bulked to 40mL using 2% HNO3 + 0.5% HClv/v
DISCUSSION
- A minor of amount of material settled on the bottom of the tube after bulking
- Samples were filtered prior to analysis
BRAN-1 is a Reference Material prepared from refined corn bran obtained from A.E Staley Manufacturing Co., Decatur, IL USA. BRAN-1, formerly known as RM 8433 was prepared by the Centre for Land and Biological resources Research, Agriculture Canada. BRAN-1; Corn Bran; Reference Material; National Research Council Canada; Ottawa, Ontario Canada (September 2015)
Results
|
NRC BRAN-1 (Corn Bran) |
||||||||||
|
Method: |
0.5g |
15mL HNO3 + 2mL H2O2 Digest at 60% power for 10 minutes. |
||||||||
|
Element |
Reference Value (mg/kg) |
95% Confidence Limits |
Sample A |
Sample B |
Sample C |
Average (ppm) |
Stdev |
% RSD |
% Recovery |
|
|
Low |
High |
|||||||||
|
Ca |
420 |
382 |
458 |
326 |
486 |
461 |
424 |
70.349 |
16.6% |
101% |
|
Cd |
0.012 |
0.007 |
0.017 |
0.011 |
0.011 |
0.012 |
0.011 |
0.0005 |
4.2% |
94% |
|
Cu |
2.47 |
2.07 |
2.87 |
2.56 |
2.69 |
2.68 |
2.65 |
0.058 |
2.2% |
107% |
|
Fe |
14.8 |
13 |
16.6 |
15.3 |
16.5 |
15.9 |
15.9 |
0.469 |
3.0% |
107% |
|
K |
566 |
491 |
641 |
600 |
606 |
600 |
602 |
2.676 |
0.4% |
106% |
|
Mg |
818 |
759 |
877 |
802 |
781 |
786 |
789 |
9.010 |
1.1% |
97% |
|
Mn |
2.55 |
2.26 |
2.84 |
2.52 |
2.59 |
2.55 |
2.55 |
0.031 |
1.2% |
100% |
|
Na |
430 |
399 |
461 |
432 |
422 |
433 |
429 |
4.955 |
1.2% |
100% |
|
Pb |
0.14 |
0.106 |
0.174 |
0.147 |
0.17 |
0.163 |
0.16 |
0.010 |
6.0% |
114% |
|
Rb |
0.5 |
0.2 |
0.8 |
0.5 |
0.5 |
0.5 |
0.5 |
0.030 |
6.3% |
97% |
|
Se |
0.045 |
0.037 |
0.053 |
0.056 |
0.036 |
0.042 |
0.045 |
0.008 |
18.8% |
99% |
|
Sr |
4.6 |
N/A |
N/A |
5.164 |
5.24 |
5.075 |
5.2 |
0.067 |
1.3% |
112% |
|
Zn |
18.6 |
16.4 |
20.8 |
19.609 |
19.178 |
20.976 |
19.9 |
0.766 |
3.8% |
107% |
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