APPLICATION NOTE

Dogfish Liver – NRC DOLT-5

SUMMARY

The application note summarizes the digestion of DOLT-5, a Dogfish Liver Certified Reference Material using
ColdBlock™ Digestion Pro Series Technology.

Instrument:ColdBlock CBM sample digester, chiller, ICP-MS & ICP-OES
Published:March 2023
Digestion Time:10 Minutes
Acid Used:HNO3 & H2O2
Average ColdBlock Recovery vs. CRM:
  • 104% Arsenic
  • 96% Mercury
  • 107% Lead

METHODOLOGY

  1. Chiller temperature was set to -5°C
  2. 0.5g of each sample was weighed and placed into a ColdBlock™ Digestion vessel
  3. 15 mL HNO3 + 2mL H2O2 was added
  4. Sample were digested at 60% power for 10 minutes
  5. Samples were cooled and bulked to 40mL using 2% HNO3 + 0.5% HCl v/v

DISCUSSION

  • Samples were clear at the end of the digestion
  • Samples were filtered prior to analysis

Frozen dogfish liver was sourced and prepared by the Guelph Food Technology Centre in Guelph, Ontario Canada. DOLT-5, Dogfish Liver; National Research Council Canada, Ottawa, Ontario Canada, August 2014.

Results

NRC DOLT-5 (Dogfish Liver)
Method: 0.5g 15mL HNO3 + 2mL H2O2 Digest at 60% power for 10 minutes.
    Element   Reference Value (ppm) 95% Confidence Limits   Sample A   Sample B   Sample C   Average (ppm)     Stdev   % RSD   % Recovery
Low High
Ag 2.05 1.97 2.13 2.05 2.01 2.04 2.03 0.017 0.8% 99%
Al 31.7 27.5 35.9 30.9 28.5 31.9 30.4 1.423 4.7% 96%
As 34.6 32.2 37 35.7 36.3 36.4 36.1 0.292 0.8% 104%
Ca 550 470 630 558 553 553 555 2.382 0.4% 101%
Cd 14.5 13.9 15.1 14.2 13.9 14.1 14.1 0.125 0.9% 97%
Co 0.267 0.241 0.293 0.266 0.267 0.275 0.269 0.004 1.5% 101%
Cu 35 32.6 37.4 36 36 36 36 0.213 0.6% 103%
Fe 1070 990 1150 1086 1063 1053 1067 14.116 1.3% 100%
Hg 0.44 0.26 0.62 0.43 0.40 0.44 0.42 0.017 4.0% 96%
K 14400 11400 17400 14329 14293 14220 14281 45.007 0.3% 99%
Mg 940 840 1040 948 941 932 940 6.565 0.7% 100%
Mn 8.91 8.21 9.61 9.73 9.32 8.99 9.35 0.303 3.2% 105%
Mo 1.41 1.19 1.63 1.45 1.48 1.48 1.47 0.014 1.0% 104%
Na 9900 8300 11500 9904 9907 9777 9862 60.695 0.6% 100%
P 11500 N/A N/A 11360 11474 11139 11324 138.766 1.2% 98%
Pb 0.162 0.13 0.194 0.172 0.167 0.183 0.174 0.007 3.8% 107%
Sb 0.013 N/A N/A 0.016 0.018 0.016 0.017 0.001 5.7% 128%
Se 8.3 6.5 10.1 8.3 8.3 8.3 8.3 0.024 0.3% 100%
Sn 0.069 0.033 0.105 0.064 0.066 0.075 0.068 0.005 7.0% 99%
Sr 3.73 3.47 3.99 3.68 3.67 3.74 3.70 0.031 0.8% 99%
Tl 0.013 N/A N/A 0.013 0.013 0.014 0.013 0.0005 3.5% 103%
U 0.082 N/A N/A 0.078 0.076 0.077 0.077 0.001 1.1% 94%
V 0.51 0.45 0.57 0.50 0.59 0.57 0.55 0.042 7.5% 109%
Zn 105.3 99.9 110.7 105.7 105.8 105.7 105.8 0.080 0.1% 100%

Click here to download the Application Note in PDF format.

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