Dogfish Liver - NRC DOLT-5 - ColdBlock Technologies

APPLICATION NOTE

Dogfish Liver – NRC DOLT-5

SUMMARY

The application note summarizes the digestion of DOLT-5, a Dogfish Liver Certified Reference Material using
ColdBlock Digestion Pro Series Technology.

Instrument:	ColdBlock CBM sample digester, chiller, ICP-MS & ICP-OES
Published:	March 2023
Digestion Time:	10 Minutes
Acid Used:	HNO₃ & H₂O₂
Average ColdBlock Recovery vs. CRM:	104% Arsenic 96% Mercury 107% Lead

METHODOLOGY

Chiller temperature was set to -5°C
0.5g of each sample was weighed and placed into a ColdBlock Digestion vessel
15 mL HNO₃ + 2mL H₂O₂ was added
Sample were digested at 60% power for 10 minutes
Samples were cooled and bulked to 40mL using 2% HNO3 + 0.5% HCl _v/v

DISCUSSION

Samples were clear at the end of the digestion
Samples were filtered prior to analysis

Frozen dogfish liver was sourced and prepared by the Guelph Food Technology Centre in Guelph, Ontario Canada. DOLT-5, Dogfish Liver; National Research Council Canada, Ottawa, Ontario Canada, August 2014.

Results

NRC DOLT-5 (Dogfish Liver)
Method:	0.5g	15mL HNO3 + 2mL H2O2 Digest at 60% power for 10 minutes.
Element	Reference Value (ppm)	95% Confidence Limits		Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
Element	Reference Value (ppm)	Low	High	Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
Ag	2.05	1.97	2.13	2.05	2.01	2.04	2.03	0.017	0.8%	99%
Al	31.7	27.5	35.9	30.9	28.5	31.9	30.4	1.423	4.7%	96%
As	34.6	32.2	37	35.7	36.3	36.4	36.1	0.292	0.8%	104%
Ca	550	470	630	558	553	553	555	2.382	0.4%	101%
Cd	14.5	13.9	15.1	14.2	13.9	14.1	14.1	0.125	0.9%	97%
Co	0.267	0.241	0.293	0.266	0.267	0.275	0.269	0.004	1.5%	101%
Cu	35	32.6	37.4	36	36	36	36	0.213	0.6%	103%
Fe	1070	990	1150	1086	1063	1053	1067	14.116	1.3%	100%
Hg	0.44	0.26	0.62	0.43	0.40	0.44	0.42	0.017	4.0%	96%
K	14400	11400	17400	14329	14293	14220	14281	45.007	0.3%	99%
Mg	940	840	1040	948	941	932	940	6.565	0.7%	100%
Mn	8.91	8.21	9.61	9.73	9.32	8.99	9.35	0.303	3.2%	105%
Mo	1.41	1.19	1.63	1.45	1.48	1.48	1.47	0.014	1.0%	104%
Na	9900	8300	11500	9904	9907	9777	9862	60.695	0.6%	100%
P	11500	N/A	N/A	11360	11474	11139	11324	138.766	1.2%	98%
Pb	0.162	0.13	0.194	0.172	0.167	0.183	0.174	0.007	3.8%	107%
Sb	0.013	N/A	N/A	0.016	0.018	0.016	0.017	0.001	5.7%	128%
Se	8.3	6.5	10.1	8.3	8.3	8.3	8.3	0.024	0.3%	100%
Sn	0.069	0.033	0.105	0.064	0.066	0.075	0.068	0.005	7.0%	99%
Sr	3.73	3.47	3.99	3.68	3.67	3.74	3.70	0.031	0.8%	99%
Tl	0.013	N/A	N/A	0.013	0.013	0.014	0.013	0.0005	3.5%	103%
U	0.082	N/A	N/A	0.078	0.076	0.077	0.077	0.001	1.1%	94%
V	0.51	0.45	0.57	0.50	0.59	0.57	0.55	0.042	7.5%	109%
Zn	105.3	99.9	110.7	105.7	105.8	105.7	105.8	0.080	0.1%	100%

Click here to download the Application Note in PDF format.

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