APPLICATION NOTE

Bovine Muscle – NRC BOVM-1

SUMMARY

The application note summarizes the digestion of BOVM-1, a Bovine Muscle Certified Reference Material using
ColdBlock™ Digestion Pro Series Technology.

Instrument:ColdBlock CBM sample digester, chiller, ICP-MS & ICP-OES
Published:March 2023
Digestion Time:10 Minutes
Acid Used:HNO3 & H2O2
Average ColdBlock Recovery vs. CRM:
  • 111% Arsenic
  • 97% Cadmium
  • 96% Lead

METHODOLOGY

  1. Chiller temperature was set to -5°C
  2. 0.5g of each sample was weighed and  placed into a ColdBlock™ Digestion vessel
  3. 15 mL HNO3 + 2mL H2O2 was added
  4. Sample were digested at 60% power for 10 minutes
  5. Samples were cooled and bulked to 40mL using 2% HNO3 + 0.5% HClv/v

DISCUSSION

  • Digestions were mostly clear with a  inor amount of suspended lipids
  • Samples were filtered prior to analysis

BOVM-1 is a CRM prepared from Canada Grade A beef. The source of material was boneless hip, commonly denoted Canada Grade A round steak (consisting of 4 major muscles: semi-membranous, semi-tendinosis, biceps femoris and adductor).

BOVM-1, Bovine Muscle, National Research Council Canada, Ottawa, Ontario Canada, September 2015.

Results

BOVM-1 Bovine Muscle
 Method:  0.5g 15mL HNO3 + 2mL H2O2 Digest at 60% power for 10 minutes
 Element Reference Value (ppm) 95% Confidence Limits  Sample A Sample B Sample C Average (ppm) Stdev % RSD % Recovery
 low high
Al 1.7 N/A N/A 1.5 1.6 1.9 1.7 0.164 9.8% 98%
As 0.009 0.006 0.012 0.009 0.01 0.011 0.01 0.001 8.2% 111%
B 0.6 0.2 1 0.7 0.5 0.6 0.6 0.049 8.2% 100%
Ca 145 125 165 168 159 145 157 9.636 6.1% 109%
Cd 0.013 0.002 0.024 0.012 0.012 0.014 0.013 0.001 7.4% 97%
Co 0.007 0.004 0.01 0.006 0.006 0.008 0.007 0.001 14.4% 96%
Cr 0.071 N/A N/A 0.069 0.072 0.08 0.073 0.003 4.5% 102%
Cu 2.84 2.39 3.29 3.08 2.73 2.41 2.74 0.274 10.0% 96%
Fe 71.2 62 80.4 74.7 72.9 73.0 73.5 0.838 1.1% 103%
K 15200 14800 15600 14992 14873 14744 14870 101.352 0.7% 98%
Mg 960 865 1055 970 956 924 950 19.253 2.0% 99%
Mn 0.37 0.28 0.46 0.39 0.35 0.40 0.38 0.022 5.7% 103%
Mo 0.08 0.02 0.14 0.07 0.08 0.08 0.08 0.002 2.2% 94%
Na 2100 2000 2200 2156 2147 2105 2136 22.038 1.0% 102%
P 8360 7910 8810 7981 8137 8087 8068 65.229 0.8% 97%
Pb 0.38 0.14 0.62 0.35 0.36 0.38 0.36 0.012 3.4% 96%
Rb 28.7 25.2 32.2 31.1 25.6 26.1 27.6 2.491 9.0% 96%
S 8000 N/A N/A 7184 7371 7331 7295 80.143 1.1% 91%
Sb 0.01 N/A N/A 0.01 0.01 0.01 0.01 0.000 4.6% 103%
Se 0.076 0.066 0.086 0.08 0.07 0.09 0.08 0.006 7.0% 104%
Sr 0.052 0.037 0.067 0.057 0.061 0.061 0.06 0.002 3.2% 115%
Zn 142 128 156 140 149 137 142 4.734 3.3% 100%

Click here to download the Application Note in PDF format.

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