Air Dried Spinach - NRC SPIN-1 - ColdBlock Technologies

APPLICATION NOTE

Air Dried Spinach – NRC SPIN-1

SUMMARY

The application note summarizes the digestion of SPIN-1, an air-dried spinach powder Certified Reference Material using ColdBlock Digestion Pro Series Technology.

Instrument:	ColdBlock CBM sample digester, chiller & ICP-MS
Published:	March 2023
Digestion Time:	10 Minutes
Acid Used:	HNO₃ & H₂O₂
Average ColdBlock Recovery vs. CRM:	111% Arsenic 96% Cadmium 96% Lead

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METHODOLOGY

Chiller temperature was set to -5°C
0.5g of each sample was weighed and placed into a ColdBlock Digestion vessel
15 mL of HNO₃ + 2mL H₂O₂ was added
Sample was digested at 70% power for 10 minutes
Samples were cooled and bulked to 40mL using 2% HNO₃ + 0.5% HCl_v/v

DISCUSSION

Samples are mostly clear after digestion, and minor of amount of material settled on the bottom of the tube after bulking
Samples were filtered prior to analysis

SPIN-1 was prepared at the National Research Council Canada from food grade air dried spinach powder SPIN-1; Air Dried Spinach Powder; National Research Council Canada ; Ottawa, Ontario Canada (June, 2019)

Results

SPIN-1 (Air Dried Spinach Powder)
Element	Reference Value (mg/kg)	95% Confidence Limits		Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
Element	Reference Value (mg/kg)	Low	High	Sample A	Sample B	Sample C	Average (ppm)	Stdev	% RSD	% Recovery
As	0.7	0.58	0.82	0.7	0.8	0.8	0.8	0.029	3.7%	111%
Se	0.062	0.03	0.094	0.056	0.074	0.077	0.069	0.009	13.4%	111%
Cd	0.265	0.235	0.295	0.227	0.266	0.268	0.254	0.019	7.4%	96%
Pb	2.33	1.99	2.67	2.24	2.31	2.16	2.24	0.063	2.8%	96%
Sr	140.8	130.9	150.7	151.5	155.6	158.0	155.0	2.717	1.8%	110%
Zn	40.7	38.7	42.7	45.2	41.7	36.6	39.1	2.515	6.4%	96%

Click here to download the Application Note in PDF format.

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ColdBlock Technologies Inc.
4551 Zimmerman Ave
Niagara Falls, ON L2E 3M5
info@coldblock.ca.

APPLICATION NOTE