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APPLICATION NOTE

Meat Homogenate – NIST 1546a

SUMMARY

The application note summarizes the digestion of NIST 1546a, a meat homogenate standard reference material using ColdBlock Digestion Pro Series Technology

Instrument:ColdBlock CBM (with quartz test tubes), chiller, ICP-MS
Published:May 2024
Digestion Time:20 Minutes
Acid Used:HNO3& H2O2
Average ColdBlock Recovery vs. CRM:
  • 102% Copper
  • 104% Iron
  • 105% Selenium

 

METHODOLOGY

  1. Chiller temperature was set to -5°C
  2. 0.5g of each sample was weighed and placed into a quartz
    ColdBlock Digestion vessel
  3. 10 mL of HNO3
    was added and mixed with the sample
  4. Sample was digested at 65% power for 20 minutes
  5. 2mL of ≥ 30% H2O2 was added
  6. Samples were cooled and bulked to 40mL using 2% HNO3 v/v

DISCUSSION

  • Samples were digested in triplicate
  • Samples were filtered prior to analysis by ICP-MS
  • H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%, for trace analysis)
  • HNO3 (Analytichem, 250-038-175, Nitric Acid, PP, 67-70%)
  • NIST 1546a consists of a mixture of pork and chicken products
    blended in a commercial process

NIST 1546a after bulk-up to 40mL

Results

NIST 1546a – Meat Homogenate

Method:

0.5g

10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2,

let cool and bulked to 40mL with 2% HNO3 v/v

 

Element

NIST

Certified Values (mg/kg)

95% Confidence Limits

 

Sample A

 

Sample B

 

Sample C

 

Average (mg/kg)

 

Stdev

 

% RSD

 

%

Recovery

Low

High

Cu

0.605

0.554

0.656

0.671

0.532

0.656

0.620

0.062

10.1%

102%

Fe

10.17

9.82

10.52

9.86

11.06

10.29

10.40

0.50

4.8%

102%

Mg

178.1

173.3

182.9

186.6

160.8

169.3

172.2

10.8

6.2%

97%

Mn

0.286

0.262

0.31

0.272

0.257

0.270

0.266

0.007

2.5%

93%

P

1651

1619

1683

1583

1681

1806

1690

92

5.4%

102%

K

2490

2280

2700

2409

2720

2536

2555

128

5.0%

103%

Se

0.281

0.264

0.298

0.273

0.308

0.303

0.295

0.015

5.2%

105%

Na

9600

8500

10700

9677

8931

9562

9390

328

3.5%

98%

Zn

17.88

17.53

18.23

18.94

17.29

18.39

18.21

0.69

3.8%

102%

*Ba

0.077

0.058

0.096

0.078

0.071

0.065

0.071

0.005

7.4%

93%

*B

0.306

0.267

0.345

0.257

0.273

0.310

0.280

0.022

7.9%

92%

*Ca

360

230

490

334

439

366

378

44

11.6%

105%

*Mo

0.016

0.014

0.018

0.014

0.017

0.017

0.016

0.001

8.8%

100%

*Rb

2.56

2.45

2.67

2.57

2.81

2.42

2.60

0.16

6.2%

102%

*Sr

0.305

0.235

0.375

0.309

0.368

0.317

0.331

0.03

7.9%

109%

Click here to download the Application Note in PDF format.

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4551 Zimmerman Ave
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info@coldblock.ca.