Distributor login
Contact us

APPLICATION NOTE

Infant Nutritional Formula – NIST 1849b

SUMMARY

The application note summarizes the digestion of NIST 1849b, an infant nutritional formula standard reference material using ColdBlock Digestion Pro Series Technology

Instrument:ColdBlock CBM (with quartz test tubes), chiller, ICP-MS
Published:May 2024
Digestion Time:20 Minutes
Acid Used:HNO3
& H2O2
Average ColdBlock Recovery vs. CRM:
  • 101% Iron
  • 101% Molybdenum
  • 97% Selenium

 

METHODOLOGY

  1. Chiller temperature was set to -5°C
  2. 0.5g of each sample was weighed and placed into a quartz ColdBlock Digestion vessel
  3. 10 mL of HNO3 was added and mixed with the sample
  4. Sample was digested at 65% power for 20 minutes
  5. 2mL of ≥ 30% H2O2 was added
  6. Samples were cooled and bulked to 40mL using 2% HNO3 v/v

DISCUSSION

  • Samples were digested in triplicate
  • Samples were filtered prior to analysis by ICP-MS
  • H2O2 (Sigma Aldrich 95321, Hydrogen peroxide solution ≥30%,
    for trace analysis)
  • HNO3 (Analytichem 250-038-175, Nitric Acid, PP, 67-70%)
  • NIST 1849b is a milk-based, hybrid infant/adult nutritional powder, prepared by a manufacturer of infant formula and adult nutritional products

NIST 1849b after bulk-up to 40mL

Results

NIST 1849b – Adult/Infant Nutritional Formula I

Method:

0.5g

10mL HNO3 digested at 65% power for 20 minutes, then added 2mL H2O2,

let cool and bulked to 40mL with 2% HNO3 v/v

 

Element

NIST

Certified Values (mg/kg)

95% Confidence Limits

 

Sample A

 

Sample B

 

Sample C

 

Average (mg/kg)

 

Stdev

 

% RSD

 

%

Recovery

Low

High

Cr

1.033

1.015

1.051

0.913

1.005

0.926

0.948

0.041

4.3%

92%

Cu

18.96

18.66

19.26

18.93

18.69

18.29

18.64

0.3

1.4%

98%

Fe

168

160.6

175.4

174

174

161

170

6.4

3.8%

101%

Mg

1570

1540

1600

1531

1599

1518

1549

36

2.3%

99%

Mo

1.741

1.678

1.804

1.764

1.766

1.740

1.757

0.012

0.7%

101%

P

3750

3620

3880

3802

3900

3952

3885

62

1.6%

104%

Se

0.816

0.791

0.841

0.759

0.802

0.822

0.794

0.026

3.3%

97%

*Ca

5050

4830

5270

5122

4975

4805

4968

129

2.6%

98%

*Mn

46.4

44.3

48.5

44.1

42.9

43.2

43.4

0.5

1.2%

94%

*K

9014

8924

9104

9035

8723

8964

8907

133

1.5%

99%

*Na

4155

4055

4255

5127

4103

4277

4502

448

9.9%

108%

*Zn

141.9

138

145.8

161.1

147.1

138.6

148.9

9.3

6.2%

105%

Click here to download the Application Note in PDF format.

Join our monthly e-newsletter for expert tips, updates, and exclusive content delivered straight to your inbox.

ColdBlock Technologies Inc.
4551 Zimmerman Ave
Niagara Falls, ON L2E 3M5
info@coldblock.ca.